Abstract
<jats:p>The authors of the article found that a molecular genetic primer design method developed using the Primer program can be used with high efficiency on dairy farms to diagnose the BLAD genetic defect: forward F: 5'- F - TACACCCTGACTCTCTCCCA-3' and reverse R: 5'- R - AGAGAGGGGTAGGAAGGCC-3' primers. The following fragments are clearly visible on the electropherogram of heterozygous mutation carriers: 327 bp, 243 bp, and 84 bp, and the proposed method allows for high-quality genetic monitoring of animals. According to the monitoring results, 5 of the 200 Holstein cows tested were heterozygous carriers. In our study, PCR was used to detect the opportunistic pathogens Escherichia coli and Staphylococcus aureus. According to the results, the proportion of cows with chronic endometritis and postpartum endometritis in which E. coli was detected was 33.33% and 43.75%, respectively. In this group of cows, the percentage of cows with Staphylococcus aureus isolates was similar: 18.5% and 18.75%. The advantage of using PCR detection of Escherichia coli in clinical material using primers developed for the conserved region of the fimH gene is that a positive result indicates that E. coli is present in the test material and that it has the ability to form a biofilm, which provides antibiotic resistance.</jats:p>